Disruptions Beads for Biological Samples

Our 0.1mm zirconia silica beads are specifically engineered to provide effective mechanical lysis of various sample types including spores and tough tissues. Available in special reinforced 2mL polypropylene or stainless steel screw cap microvials.

Bead media composed of stainless steel or chrome-steel beads may be utilized with a dedicated Bead Beater for dry grinding (at ambient or liquid nitrogen temperatures). Use only with 2mL reinforced polypropylene or stainless steel microvials.

Density

Zirconia silica beads are relatively dense, having more weight than other materials like chrome or stainless steel. Their greater density helps them keep their shape during grinding, decreasing particle breakage and improving accuracy of finished product. Zirconia beads may also be better at grinding samples that have proven difficult to break up with other types of media.

Zirconia silica beads boast high densities that enable them to achieve precise particle sizes, making them suitable for applications in nanotechnology. Their precision allows various industries to advance through manipulating materials at both an atomic and molecular level.

Zirconia silica beads of 0.1 mm diameter can assist with creating ceramic multilayer capacitors (MLCC), an essential component in electronic devices for storing and discharging energy. Their precise, contamination-free grinding technology is instrumental to this technology by creating uniform particle distribution necessary for producing thin ceramic films needed to create these thin capacitors – further increasing performance of electronics devices.

Sharp Particles

Zirconia beads, unlike glass ones, do not break during bead beating and remain intact after bead beating. Furthermore, their sharp edge helps accelerate tissue lysis. Garnet (an iron-aluminum silicate) shares similar density but more fragile properties; during bead beating it may fracture. However, fragmentation may prove useful when trying to separate out bacteria-laden samples from grinding media.

Zirconia beads were found to produce significantly greater pollen lysis when bead beating pollen than glass beads due to alumina sandblasting, which created surface defects on zirconia beads and increased their surface roughness, increasing surface energy, and thus permitting more resin to enter these micro-retentive features of zirconia beads. This could be explained by their micro-retentive features which create micro-retention features on zirconia surfaces allowing more resin into them during bead beating pollen from glass to zirconia before beating pollen against glass beads than glass beads could do, due to surface defects from being created when hit against glass, leading to significantly greater resin retention within zirconia beads than glass ones when subject to bead beating pollen than glass ones due to being treated by sandblasting with aluminum oxide which creates surface defects on zirconia surface roughness by increased surface roughness which allowed more resin into microretentive features allowing more resin into them which allowed increased resin flow into these microretentive features allowing increased resin flow into these microretentive features allowing more resin flow into microretentive features allowing resin flow into these microretentive features that had increased surface energy increased surface energy and allowed more resin flow into these microretentive features thus encouraging resin flow into these microretentive features than previously seen with glass beads which had no such increased rough surface roughness by increasing surface roughness from its increased roughness increase surface energy increase which in turn increased resin flow into this microretentive features increasing surface energy increase surface energy increases in turn increase surface energy thus increased surface energy to flow into microretentive microretentive microretentive features and therefore increasing surface energy increasing surface energy thus increase allows flow allowing increased surface energy thus permitting resin flow into micro retentive features for resin to flow into these microretention features allowing more resin flow into these microretentive features in turn increased surface energy increase increase, increasing surface energy allowing resin flow into micro allowing more resin flow into micro allowing flow into micro allowing resin flow into micro allowing resin flowing further increasing surface energy increasing surface energy increasing surface energy increasing surface energy which allowed resin flowing more into micro allowing resin flowing into micro allowing resin flow into micro allowing flow micro allowing resin flow micro allowing resin flowing into micro allowing flow into micro allowing resin flow allowing flow thus increasing surface energy increased surface more resin flow through increasing surface energy increased surface energy opening up which so more resin flow into micro retentive features thus increasing surface energy more resin into these micro allowing resin flow micro allowing resin into these micro allowing resin into micro allowing this surface energy increase allow flow into these micro retentive features more resin flow into micro allowing resin flowing micro- allowing flow more resin flow into micro- allowing resin flow more flow into these micro allowing resin flow more resin flows into micro allowing resin entering micro allowing resin flows over time than before it had run out through thus increasing surface energy thus increasing surface energy allowed more resin flows into more flow more flow into these micro allowing more flow features thus increasing surface energy increase through increased surface energy which allowed flow more resin flow through increases surface energy increases increased surface energy making more resin flow into these micro allowing more resin flow into micro allowing flow by increasing roughness increase increased surface energy increase surface energy increases this increased surface energy allowed more resin flows that allowed more surface energy more which allows flow more resin flowing out in flow more flow- more resin flow into micro allowing flow out, increasing surface energy, increased surface

Bead beating was conducted using 2.5 mm zirconia beads and glass beads at a speed of 6.5 meters per second for 45 seconds on leaves of R. canina using bead beating rather than chopping; mean CVs for G0/G1 peak ratios were lower when the former technique was utilized, though statistical significance could not be demonstrated between these methods.

Chrome-Steel Beads

These chrome-steel beads provide aggressive mechanical lysis of tough tissues and dry plant materials like seeds and hair, due to their density of 7.9g/cc.

Beads made of Type 316 stainless steel are among the most corrosion resistant available, while electroplating adds a thin coating of inert chromium that does not contribute to sample abrasion or contamination issues. Chrome steel beads may only need to be used once before discarding, eliminating cleaning and cross-contamination issues altogether.

These heavy beads are typically employed for dry-grinding leaves and seeds, and should only be placed into special microvials with silicone rubber caps or reinforced polypropylene microvials containing silicon reinforcement, because ordinary screw-cap polypropylene vials would likely crack under their weight or leak too easily.

Garnet sharp particles (an iron-aluminum silicate) differ from less costly glass beads and zirconia silica beads in that they fragment during beadbeating, possibly acting faster on tough samples than other sharp particles. Furthermore, Garnet sharp particles may even be useful in cell lysis applications.

Stainless Steel Beads

Stainless steel beads make an effective replacement for glass or chrome-steel media when blasting off heavy corrosion from parts or surfaces, as they are harder and much less likely to shatter under impact than glass media. Furthermore, being reusable decreases frequency of medium replacement and production costs.

Bead blasting with Yttria-stabilized zirconia bead blasting is an effective way to remove stains and burrs from aluminum alloy parts to enhance their strength, finish quality and fatigue resistance. Furthermore, ceramic surfaces can also benefit from being polished using this process, giving better surface smoothness and wear resistance.

Zirconium silicate has similar density to glass, making it suitable for homogenizing most types of tissues and spores. Due to its durability, precision (roundness), and moderate cost it is often chosen as the medium for disrupting soft tissues or bead-beating bacteria-containing samples in stirred mills; furthermore it stands up well under vigorous agitation from stirred mills; however it should not be used when homogenizing tough fibrous plant material like monocotyledon leaves.

Tungsten Carbide Beads

Tungsten carbide beads are zirconia silicate beads containing tungsten that are typically used to make welds from this metal and often coated on nickel alloys to increase their hardness and improve weld strength.

Tungsten carbide beads can also help to protect metal parts against corrosion and in applications requiring them to cut hard materials like stone or concrete. Furthermore, they’re ideal for weldments where stress levels are particularly high.

Tungsten carbide-coated beads help improve weldability by lowering the melting point of nickel alloy, leading to stronger bonds between cladding and base metal. Furthermore, their iron levels remain at an acceptable level to maximize weldability.

Zirconium silicate beads are an ideal choice for sample disruption with the TissueLyser system, as they’re suitable for both wet and dry protocols. Available in multiple sizes with both wheel and compressed air systems for use, these off-white-colored beads feature a shiny surface.